In silico analysis of drug resistance in wild type and mutant HIV-1 subtype d protease
نویسندگان
چکیده
Methods The mutation frequency of subtype d in untreated persons was obtained from Stanford DR database, http://hivdb. stanford.edu/. Based on the database the wild type PTD sequence was generated. Crystal structure 3LZS showed more similarity based on BLASTp program. The protein structure 3LZS was used as a template to build wild type, major (L10V, N37D, K69Y), minor (K20I, L33I, P39T, Q61N), major+minor mutants of PTD using Modeller9v7. The docking studies of protease inhibitors (atazanavir, darunavir, indinavir, lopinavir, nelfinavir, ritonavir, saquinavir, tipranavir) with the wild type and mutant models were carried using AutoDock 4.2.5.
منابع مشابه
Resistance mechanism of human immunodeficiency virus type-1 protease to inhibitors: A molecular dynamic approach
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